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We began analyzing https://www.nature.com/articles/4400361, but it redirected us to https://www.nature.com/articles/4400361. The analysis below is for the second page.

Title[redir]:
An insertional mutagenesis approach to Dictyostelium cell death | Cell Death & Differentiation
Description:
Programmed cell death (PCD) in Dictyostelium shows a pattern of ordered degeneration similar to that observed in higher eukaryotes but somewhat different from the most studied form of PCD, i.e. apoptosis. To contribute to a genetic definition of this process, Dictyostelium HMX44A cells have been subjected to insertional mutagenesis, followed by selection based on several rounds of differentiation/regrowth to recover only cells resistant to death. We describe here the approach used, a partial characterization of the first mutant thus obtained called C5 showing some dissociation of cell death signs, and, in this case where plasmid rescue was not possible, as a first step towards identification of the gene at play recovery of genomic flanking sequences via genomic recircularization and PCR. This work demonstrates the feasibility of an insertional mutagenesis approach to obtain death-resistant mutants in Dictyostelium.

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Keywords {🔍}

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Topics {✒️}

nature portfolio permissions reprints nature programmed cell death obtain death-resistant mutants dictyostelium cell death author correspondence cell death signs cell death differ insertional mutagenesis approach dictyostelium hmx44a cells cell death similar content olie & pierre golstein explore content model organisms permissions insertional mutagenesis journals search log homology-directed insertion developmentally regulated genes gene knockout cells article cornillon ordered degeneration similar genomic flanking sequences crispr-based engineering content melino rights stress extrusion article 05 differentiation/regrowth pierre golstein article cite cells resistant dictyostelium shows cookies article differentiation death marseille selection based genomic recircularization dictyostelium journal publish higher eukaryotes studied form genetic definition partial characterization plasmid rescue play recovery

Questions {❓}

Cell death in Leishmania induced by stress and differentiation: programmed cell death or necrosis?

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WebPage:
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      headline:An insertional mutagenesis approach to Dictyostelium cell death
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