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BIGSdb: Scalable analysis of bacterial genome variation at the population level | BMC Bioinformatics | Full Text
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Background The opportunities for bacterial population genomics that are being realised by the application of parallel nucleotide sequencing require novel bioinformatics platforms. These must be capable of the storage, retrieval, and analysis of linked phenotypic and genotypic information in an accessible, scalable and computationally efficient manner. Results The Bacterial Isolate Genome Sequence Database (BIGSDB) is a scalable, open source, web-accessible database system that meets these needs, enabling phenotype and sequence data, which can range from a single sequence read to whole genome data, to be efficiently linked for a limitless number of bacterial specimens. The system builds on the widely used mlstdbNet software, developed for the storage and distribution of multilocus sequence typing (MLST) data, and incorporates the capacity to define and identify any number of loci and genetic variants at those loci within the stored nucleotide sequences. These loci can be further organised into
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springer nature home page[http implementation bigsdb jolleyย &ย martin cj maiden methicillin-resistant staphylococcus aureus availability enabling fine-grained control multi-locus sequence typing main index page author information authors open access article client-side javascript makes main results tables tab-delimited text format authors scientific editing multilocus sequence typing factor h-binding protein web-accessible database system markowitz vm short-read solexa data fine-grained authentication results design philosophy conserved genomic sequence high-quality reference genome standard query interface cross-reference typing methods privacy choices/manage cookies identifying gene-length regions allele content continents reveals hypervirulent article jolley authorsโ original file extended-fasta format suitable reference gene-based analysis specimen-centric view required multilocus typing data illumina solexa reads existing genomic methods //perl-md5-login define variable regions multilocus sequence data high-throughput genome sequencing allowing additional features client-side javascript extended sequence typing laboratory samples disease management multi-fasta format existing sequence typing org/software/database/bigsdb/
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headline:BIGSdb: Scalable analysis of bacterial genome variation at the population level
description:The opportunities for bacterial population genomics that are being realised by the application of parallel nucleotide sequencing require novel bioinformatics platforms. These must be capable of the storage, retrieval, and analysis of linked phenotypic and genotypic information in an accessible, scalable and computationally efficient manner. The Bacterial Isolate Genome Sequence Database (BIGSDB) is a scalable, open source, web-accessible database system that meets these needs, enabling phenotype and sequence data, which can range from a single sequence read to whole genome data, to be efficiently linked for a limitless number of bacterial specimens. The system builds on the widely used mlstdbNet software, developed for the storage and distribution of multilocus sequence typing (MLST) data, and incorporates the capacity to define and identify any number of loci and genetic variants at those loci within the stored nucleotide sequences. These loci can be further organised into 'schemes' for isolate characterisation or for evolutionary or functional analyses. Isolates and loci can be indexed by multiple names and any number of alternative schemes can be accommodated, enabling cross-referencing of different studies and approaches. LIMS functionality of the software enables linkage to and organisation of laboratory samples. The data are easily linked to external databases and fine-grained authentication of access permits multiple users to participate in community annotation by setting up or contributing to different schemes within the database. Some of the applications of BIGSDB are illustrated with the genera Neisseria and Streptococcus. The BIGSDB source code and documentation are available at
http://pubmlst.org/software/database/bigsdb/
. Genomic data can be used to characterise bacterial isolates in many different ways but it can also be efficiently exploited for evolutionary or functional studies. BIGSDB represents a freely available resource that will assist the broader community in the elucidation of the structure and function of bacteria by means of a population genomics approach.
datePublished:2010-12-10T00:00:00Z
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Allele Sequence
Laboratory Information Management System
Query Interface
Population Genomic
Bioinformatics
Microarrays
Computational Biology/Bioinformatics
Computer Appl. in Life Sciences
Algorithms
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headline:BIGSdb: Scalable analysis of bacterial genome variation at the population level
description:The opportunities for bacterial population genomics that are being realised by the application of parallel nucleotide sequencing require novel bioinformatics platforms. These must be capable of the storage, retrieval, and analysis of linked phenotypic and genotypic information in an accessible, scalable and computationally efficient manner. The Bacterial Isolate Genome Sequence Database (BIGSDB) is a scalable, open source, web-accessible database system that meets these needs, enabling phenotype and sequence data, which can range from a single sequence read to whole genome data, to be efficiently linked for a limitless number of bacterial specimens. The system builds on the widely used mlstdbNet software, developed for the storage and distribution of multilocus sequence typing (MLST) data, and incorporates the capacity to define and identify any number of loci and genetic variants at those loci within the stored nucleotide sequences. These loci can be further organised into 'schemes' for isolate characterisation or for evolutionary or functional analyses. Isolates and loci can be indexed by multiple names and any number of alternative schemes can be accommodated, enabling cross-referencing of different studies and approaches. LIMS functionality of the software enables linkage to and organisation of laboratory samples. The data are easily linked to external databases and fine-grained authentication of access permits multiple users to participate in community annotation by setting up or contributing to different schemes within the database. Some of the applications of BIGSDB are illustrated with the genera Neisseria and Streptococcus. The BIGSDB source code and documentation are available at
http://pubmlst.org/software/database/bigsdb/
. Genomic data can be used to characterise bacterial isolates in many different ways but it can also be efficiently exploited for evolutionary or functional studies. BIGSDB represents a freely available resource that will assist the broader community in the elucidation of the structure and function of bacteria by means of a population genomics approach.
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Multilocus Sequence Typing
Allele Sequence
Laboratory Information Management System
Query Interface
Population Genomic
Bioinformatics
Microarrays
Computational Biology/Bioinformatics
Computer Appl. in Life Sciences
Algorithms
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